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恩諾沙星及其代謝物在方正銀鲫組織中的代謝及消除規律

點擊數:2400  時間:2019-07-31 14:10:39  來源: 中國動物檢疫      

近年來水産養殖中的常用藥物恩諾沙星在鲫中的殘留問題比較突出,但目前國家未對其在鲫中的休藥期作出明确規定。為做到鲫養殖中科學合理使用恩諾沙星,在12~15 ℃水溫條件下,以60 mg/kg.b.w.劑量,對體質量為(250±10)g的健康方正銀鲫(Carassius auratus gibelio)灌服恩諾沙星;在灌服後0~120 d内不間斷采樣,用高效液相色譜-串聯質譜儀檢測,研究恩諾沙星及其代謝産物環丙沙星在方正銀鲫鰓、血漿、肌肉、皮膚、肝髒和腎髒中的代謝及消除規律,從而為恩諾沙星在方正銀鲫中的休藥期制定提供參考。結果顯示:灌藥後,恩諾沙星在方正銀鲫血漿、肌肉、肝髒、鰓、腎髒和皮膚中的達峰時間tmax分别為9、18、24、24、48和48 h,腎髒、肝髒、肌肉、血漿、鰓和皮膚中的達峰濃度cmax分别為30.490、21.372、18.715、16.636、15.157和11.663mg/kg;皮膚中的消除半衰期t1/2β最大,為338.2 h,血漿中最小,為93.303 h;代謝産物環丙沙星的代謝及消除趨勢與恩諾沙星大緻相同,血漿中達峰值産生最早,tmax為18 h,腎髒中的峰濃度最大,cmax為547.26 μg/kg。結果表明,方正銀鲫以單次口灌60 mg/kg.b.w.劑量恩諾沙星,皮膚和肌肉中的恩諾沙星和環丙沙星總量需要1200度日才能滿足限量要求。本研究為恩諾沙星在鲫魚養殖中的科學使用提供了數據支撐。

Establishmentof a Real-time RPA Method for Rapid Detection of Lymphocytic ChoriomeningitisVirus

Inrecent years,the residues of Enrofloxacin that has been commonly used inaquaculture have become more serious in Carassius auratus gibelio,however,no any clear regulation on itswithdrawal period has been developed by the state. In order to scientificallyuse the Enrofloxacin in carp breeding,the followingexperiment was carried out. Healthy Carassius auratus gibelio with body mass of(250±10)g was fedwith Enrofloxacin at a dose of 60 mg/kg.b.w. under the water temperature of 12to 15 ℃,and then incessantly sampled within 0 to 120 dto test by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS),hence the metabolism mechanism wasstudied,as well as the elimination rule of Enrofloxacinand its metabolite(Ciprofloxacin)in gill,plasma,muscle,skin,liver and kidney,which could provide some references for defining its withdrawalperiod. The results showed that,after administration,the peak time(tmax)ofEnrofloxacin in plasma,muscle,liver,gill,kidney and skin was 9,18,24,24,48 and 48 h,respectively and the peakconcentration(cmax)was 16.636,18.715,21.372,15.157,30.490 and 11.663 mg/kg,respectively. Theelimination half-life(t1/2β)inskin was the maximum,which was 338.2 h,and in plasma it was the minimum(93.303 h). The metabolism and elimination trend of Ciprofloxacin was similarto that of Enrofloxacin,specially,the peak value first appeared in plasma with tmax of 18 h,and the peak concentration reached the maximum in kidney with cmaxof 547.26 μg/kg. Therefore,aconclusion was given that it would take more than 1 200 degree-days for thetotal amount of Enrofloxacin and Ciprofloxacin in skin and muscle to reach therequired limit if Carassius auratus gibelio was orally administered withEnrofloxacin at a dose of 60 mg/kg.b.w each time. Therefore,some data supports were provided by this study for scientific use ofEnrofloxacin in carp breeding.

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